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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
24/04/2018 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIANNITTI, F.; FRAGA, M.; CAFFARENA, D.; SCHILD, C.; BANCHERO, G.; ARMIÉN, A.G.; TRAVERÍA, G.; MARTHALER, D.; WELLS, S.J.; RIET-CORREA, F. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANÍBAL GUILLERMO ARMIÉN, Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.; GABRIEL TRAVERÍA, Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE), Universidad Nacional de La Plata, Chascomús, Buenos Aires, Argentina.; DOUGLAS MARTHALER, Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.; SCOTT JOSEPH WELLS, Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Mycobacterium paratuberculosis sheep type strain in Uruguay: Evidence for a wider geographic distribution in South America. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Journal of Infection in Developing Countries , v. 12, n. 3, p.190-195, 2018. |
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DOI : |
10.3855/jidc.9751 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 11 September 2017 // Accepted 15 March 2018. |
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Contenido : |
Abstract:
Johne?s disease (JD) is an economically important disease of ruminants caused by Mycobacterium avium paratuberculosis (MAP), which also
infects other species including humans. Two major MAP strain types are currently recognized: sheep (S) and cattle (C) types. Information on
JD prevalence and MAP types infecting small ruminants in South America is limited, and all but one of the MAP types reported from this
region are of the C type. This study describes clinicopathological, molecular and microbiological findings in 11 cases of JD caused by a type
S MAP strain, and estimated true within-flock prevalence in a ~735-sheep operation in Uruguay. Postmortem examination and histology
(hematoxylin-eosin and Ziehl-Neelsen stains) of samples from 41 selected sheep revealed lymphohistiocytic/granulomatous enteritis and
mesenteric lymphadenitis in 11 animals, with moderate/severe multibacillary lesions in 6 clinical cases, and minimal/mild paucibacillary lesions
in 5 sub-clinical cases. Immunohistochemistry using an antibody against Mycobacterium bovis that cross-reacts with MAP (2 cases), and
transmission electron microscopy (1 case), revealed myriads of intrahistiocytic mycobacteria. MAP was isolated in one case and detected by
PCR in 6 cases. The S type of MAP was identified using a multiplex PCR that distinguishes between S and C types, and PCR-REA. The
estimated true within-flock prevalence was ? 2.3%. This represents the first communication on within-flock prevalence of JD associated with
a type S MAP strain in South America and the second documentation of this strain in the subcontinent. Additional studies are required to better
understand the molecular epidemiology of the different MAP types in the region. MenosAbstract:
Johne?s disease (JD) is an economically important disease of ruminants caused by Mycobacterium avium paratuberculosis (MAP), which also
infects other species including humans. Two major MAP strain types are currently recognized: sheep (S) and cattle (C) types. Information on
JD prevalence and MAP types infecting small ruminants in South America is limited, and all but one of the MAP types reported from this
region are of the C type. This study describes clinicopathological, molecular and microbiological findings in 11 cases of JD caused by a type
S MAP strain, and estimated true within-flock prevalence in a ~735-sheep operation in Uruguay. Postmortem examination and histology
(hematoxylin-eosin and Ziehl-Neelsen stains) of samples from 41 selected sheep revealed lymphohistiocytic/granulomatous enteritis and
mesenteric lymphadenitis in 11 animals, with moderate/severe multibacillary lesions in 6 clinical cases, and minimal/mild paucibacillary lesions
in 5 sub-clinical cases. Immunohistochemistry using an antibody against Mycobacterium bovis that cross-reacts with MAP (2 cases), and
transmission electron microscopy (1 case), revealed myriads of intrahistiocytic mycobacteria. MAP was isolated in one case and detected by
PCR in 6 cases. The S type of MAP was identified using a multiplex PCR that distinguishes between S and C types, and PCR-REA. The
estimated true within-flock prevalence was ? 2.3%. This represents the first communication on within-flock prevalence of J... Presentar Todo |
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Palabras claves : |
ENTERITIS; INFECTIOUS DISEASE; LIVESTOCK; PARATUBERCULOSIS; PLATAFORMA SALUD ANIMAL; RUMINANTS. |
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Thesagro : |
ENFERMEDADES INFECCIOSAS; OVEJA; RUMIANTES; URUGUAY. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02872naa a2200373 a 4500
001 1058474
005 2019-10-09
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3855/jidc.9751$2DOI
100 1 $aGIANNITTI, F.
245 $aMycobacterium paratuberculosis sheep type strain in Uruguay$bEvidence for a wider geographic distribution in South America.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 11 September 2017 // Accepted 15 March 2018.
520 $aAbstract: Johne?s disease (JD) is an economically important disease of ruminants caused by Mycobacterium avium paratuberculosis (MAP), which also infects other species including humans. Two major MAP strain types are currently recognized: sheep (S) and cattle (C) types. Information on JD prevalence and MAP types infecting small ruminants in South America is limited, and all but one of the MAP types reported from this region are of the C type. This study describes clinicopathological, molecular and microbiological findings in 11 cases of JD caused by a type S MAP strain, and estimated true within-flock prevalence in a ~735-sheep operation in Uruguay. Postmortem examination and histology (hematoxylin-eosin and Ziehl-Neelsen stains) of samples from 41 selected sheep revealed lymphohistiocytic/granulomatous enteritis and mesenteric lymphadenitis in 11 animals, with moderate/severe multibacillary lesions in 6 clinical cases, and minimal/mild paucibacillary lesions in 5 sub-clinical cases. Immunohistochemistry using an antibody against Mycobacterium bovis that cross-reacts with MAP (2 cases), and transmission electron microscopy (1 case), revealed myriads of intrahistiocytic mycobacteria. MAP was isolated in one case and detected by PCR in 6 cases. The S type of MAP was identified using a multiplex PCR that distinguishes between S and C types, and PCR-REA. The estimated true within-flock prevalence was ? 2.3%. This represents the first communication on within-flock prevalence of JD associated with a type S MAP strain in South America and the second documentation of this strain in the subcontinent. Additional studies are required to better understand the molecular epidemiology of the different MAP types in the region.
650 $aENFERMEDADES INFECCIOSAS
650 $aOVEJA
650 $aRUMIANTES
650 $aURUGUAY
653 $aENTERITIS
653 $aINFECTIOUS DISEASE
653 $aLIVESTOCK
653 $aPARATUBERCULOSIS
653 $aPLATAFORMA SALUD ANIMAL
653 $aRUMINANTS
700 1 $aFRAGA, M.
700 1 $aCAFFARENA, D.
700 1 $aSCHILD, C.
700 1 $aBANCHERO, G.
700 1 $aARMIÉN, A.G.
700 1 $aTRAVERÍA, G.
700 1 $aMARTHALER, D.
700 1 $aWELLS, S.J.
700 1 $aRIET-CORREA, F.
773 $tJournal of Infection in Developing Countries$gv. 12, n. 3, p.190-195, 2018.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
08/06/2021 |
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Actualizado : |
02/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GASTAL, G.D.A.; SCARLET D.; MELCHERT M.; ERTL R.; AURICH, C. |
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Afiliación : |
GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; DRAGOS SCARLET, Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;; MARIA MELCHERT, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; REINHARD ERT, Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; CHRISTINE AURICH, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. |
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Título : |
Epigenetic changes in equine embryos after short-term storage at different temperaturas. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Animals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325 |
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DOI : |
10.3390/ani11051325 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria. |
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Contenido : |
Simple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim
of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development,
maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C);
day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation
(malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There
were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis
(BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and
DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest
some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer. MenosSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and... Presentar Todo |
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Palabras claves : |
Development; Embryo; Embryo-maternal recognition; Equine; Methylation; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Transport. |
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Thesagro : |
EQUINOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16671/1/animals-11-01325.pdf
https://www.mdpi.com/2076-2615/11/5/1325/pdf
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Marc : |
LEADER 04973naa a2200301 a 4500
001 1062116
005 2022-09-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/ani11051325$2DOI
100 1 $aGASTAL, G.D.A.
245 $aEpigenetic changes in equine embryos after short-term storage at different temperaturas.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria.
520 $aSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer. Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C); day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation (malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis (BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer.
650 $aEQUINOS
653 $aDevelopment
653 $aEmbryo
653 $aEmbryo-maternal recognition
653 $aEquine
653 $aMethylation
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTransport
700 1 $aSCARLET D.
700 1 $aMELCHERT M.
700 1 $aERTL R.
700 1 $aAURICH, C.
773 $tAnimals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325
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